说明：是一种天然多糖，取自猪或牛肠粘液或牛肺，用于生化研 究，防止凝血酶原转变成凝血酶。科研上常用于标签蛋白的切割。
别名：肝素；肝磷脂；Heparinar；Hepsal；Lipohepin
CAS＃：9041-08-1
外观：白色或类白色粉末
特性：纯度：＞99％            from bovine plasma 
          效价：≥200U/mg
           溶解性：溶于水，参考浓度50mg/ml。
储存条件： 2-8℃避光

Thrombin cleavage

1. Thrombin cleavage of eluted fusion protein bound to Sepharose
* Mix 50 μl of thrombin (< 10 cleavage U/ml) solution and 950 μl of 1 x PBS for each ml of Glutathione Sepharose bed volume
Add thrombin protease mixture to Glutathione Sepharose pellet
Gently shake or rotate the suspension at r.t. for 2-16 h
Centrifuge the suspension at 500 x g for 5’ to pellet the beads and carefully transfer the eluted fraction to a clean tube.
2. Thrombin cleavage of eluted fusion protein.
Add 10 μl of thrombin solution (10 cleavage units) per mg fusion protein. If the amount of fusion protein in the eluate has not been determined, add 80 μl (80 U) of thrombin for each ml of Glutathione Sepharose bed volume from with the fusion protein was eluted.
Mix gently and incubate at r.t. (22-25C) for 2-16 h.
Once digestion is complete, GST can be removed by first removing glutathione by extensive dialysis (2,000 vol/ml) against 1 x PBS followed by batch purification.